Kinetic Studies of Levansucrase of Bacillus subtilis
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چکیده
منابع مشابه
Secretion of Bacillus subtilis levansucrase
The refolding of levansucrase denatured by urea was studied as a possible model for the second step of the secretion pathway of this protein. The folding-unfolding transition was monitored by measuring intrinsic fluorescence and resistance to proteolysis. Both methods provided the same estimation for the unfolding free energy of levansucrase, AGD, which was 30.1 + 1.7 kJ mol-1 (7.2 + 0.4 kcal m...
متن کاملIMMOBILISATION OF Bacillus subtilis NRC33a LEVANSUCRASE AND SOME STUDIES ON ITS PROPERTIES
Bacillus subtilis NRC33a levansucrase was immobilised on different carriers using different immobilisation methods including physical adsorption, covalent binding, ionic binding and entrapment. The immobilised enzyme prepared by covalent binding on chitosan through 3% gluteraldehyde had the highest immobilization yield (81.51%). Therefore, it was used as a typical example for Bacillus subtilis ...
متن کاملHyperproduction of exocellular levansucrase by Bacillus subtilis: examination of the phenotype of a sacUh strain.
The induction parameters of levansucrase synthesis were the same in Bacillus subtilis strain 168 Marburg and in a derivative, hyperproducing (sacUh) strain. However, only the hyperproducing strain showed an induction lag period. The kinetics of appearance of functional levansucrase mRNA was established. Strain 168 did not release levansucrase, but washing the cells with high ionic strength buff...
متن کاملIncreasing the stability of sacB transcript improves levansucrase production in Bacillus subtilis.
AIMS To develop a strategy to increase the stability of transcripts of structural genes expressed under the control of sacR, the leader region of Bacillus subtilis levansucrase gene. METHODS AND RESULTS Insertion of Shine Dalgarno like sequences in the 5'-untranslated sacR region controlling the expression of sacB. Depending on the number of stabilizing sequences inserted and the position of ...
متن کاملPhysiological studies of Bacillus subtilis minicells.
Minicells produced by Bacillus subtilis strains carrying the div IV-B1 mutation, (CU 403 div IV-B1 and CU 403 div IV-B1, tag-1), were purified by a procedure which destroys parental cells with ultrasound, but spares minicells. Such preparations generally contain 10(9) or more minicells/ml and less than 10(4) colony-forming units/ml. Purified minicells are resistant to autolysis in tris(hydroxym...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1974
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1974.tb03269.x